Nights are playable Levels of the game, if the player complete all Nights player will "win" the game.
Night 1 is the first playable night of Five Nights at Treasure Island. Compared to the later nights, this night is relatively easy and serves mainly as an introductory stage that accustoms the player to the game's mechanics, allowing the player (if new) to get used to how to survive, and to get use to the building's whereabouts.
Night 2 is the second playable night of Five Nights at Treasure Island, as well as the last playable night in the old demo. This night starts immediately increasing the difficulty, as Photo-Negative Mickey will start to get more active and Oswald becomes a threat from this point onward. Disembodied and The Face are also active this night and one of them can also sometimes appear in the Office, effectively multiplying the number of potential threats to watch out for.
Night 3 is the third night of Five Nights at Treasure Island. Unlike the previous two nights this night is cut short to 3AM, which the player will be sent to Pirate Caverns, which that is where the Player will have to survive against The Face and Undying. Photo-Negative Mickey and Oswald are more aggressive than they were in the previous night. The Face and Disembodied also have a higher chance of entering the Office this night compare to the previous night. Acephalous, Suicide Mouse will be active on this night as well. On rare cases Pluto may enter the Office. In the remastered version, Hourglass will be active along with Acephalous, Suicide Mouse.
The Pirate Caverns is a special area accessed as the second half of Night 3. This area is where Undying will start. When the player enters Pirate Caverns, the game style changes to a point-and-click type of adventure game where the player clicks to go to other areas in the cavern. The player can enable a flashlight by pressing the space bar. The Face will also be a threat in this area.
Night 4 is one of the nights in Five Nights at Treasure Island, which unlike Night 3, this Night will not End at 3AM, Night 3 was the only night to end at 3AM. This night drastically increases the difficulty as most of the characters become active at this point. Acephalous, Suicide Mouse, Hourglass(In remastered version) are much more active this night as well as all the previous suits. Photo-Negative Minnie and Undying are also active this night. The player must also constantly check Meat Freezer sometimes to see Undying. If he in office you need Shut off a cam or power. In remastered version, Ortensia, Daisy Duck will be active along with Photo-Negative Minnie.
Active suits on this night are:
Photo-Negative Mickey, Oswald, Disembodied, The Face, Suicide Mouse, Pluto, Acephalous, Undying, Photo Negative Minnie. Henry, MickMick are also active on this night. In remastered version active on this night are:
More harder then Night 5.
Active Suits on this night are:
In remastered version, only Past Mickey is active.
Five Nights at Treasure Island Remastered
This night only playable in Remastered version.
Active Suits on this night are:
Infinite Night will be a never-ending night where the Suits and Humanoids AI difficulty will get higher as the night goes on. As suggested by the name, the night will never end. This is a mode where the player must try and survive as long as possible to get a high score. This mode only playable in Remastered Version.